全文获取类型
收费全文 | 380903篇 |
免费 | 25121篇 |
国内免费 | 2818篇 |
学科分类
医药卫生 | 408842篇 |
出版年
2021年 | 2459篇 |
2019年 | 2771篇 |
2018年 | 4572篇 |
2017年 | 3478篇 |
2016年 | 3580篇 |
2015年 | 4087篇 |
2014年 | 5785篇 |
2013年 | 7494篇 |
2012年 | 10167篇 |
2011年 | 10415篇 |
2010年 | 6305篇 |
2009年 | 5948篇 |
2008年 | 9634篇 |
2007年 | 10470篇 |
2006年 | 10391篇 |
2005年 | 9458篇 |
2004年 | 8996篇 |
2003年 | 8740篇 |
2002年 | 8395篇 |
2001年 | 28360篇 |
2000年 | 28889篇 |
1999年 | 23715篇 |
1998年 | 5075篇 |
1997年 | 4151篇 |
1996年 | 3716篇 |
1995年 | 3510篇 |
1994年 | 3138篇 |
1993年 | 2880篇 |
1992年 | 16132篇 |
1991年 | 14902篇 |
1990年 | 14243篇 |
1989年 | 14023篇 |
1988年 | 12652篇 |
1987年 | 12122篇 |
1986年 | 11157篇 |
1985年 | 10381篇 |
1984年 | 6952篇 |
1983年 | 5639篇 |
1982年 | 2733篇 |
1979年 | 5512篇 |
1978年 | 3364篇 |
1977年 | 2992篇 |
1975年 | 2656篇 |
1974年 | 3097篇 |
1973年 | 2886篇 |
1972年 | 2849篇 |
1971年 | 2795篇 |
1970年 | 2523篇 |
1969年 | 2560篇 |
1968年 | 2270篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
51.
D.M.L. Saunte B.M. Piraccini A.Y. Sergeev A. Prohi B. Sigurgeirsson C. Rodríguez‐Cerdeira J.C. Szepietowski J. Faergemann M. Arabatzis M. Pereiro M. Skerlev P. Lecerf P. Schmid‐Grendelmeier P. Nenoff R.J. Nowicki L. Emtestam R.J. Hay 《Journal of the European Academy of Dermatology and Venereology》2019,33(2):421-427
52.
Merkel cell carcinoma (MCC) is a rare neuroendocrine carcinoma of the skin, for which the exact mechanisms of carcinogenesis remain unknown. Therapeutic options for this highly aggressive malignancy have historically been limited in both their initial response and response durability. Recent improvements in our understanding of MCC tumor biology have expanded therapeutic options for these patients, namely through the use of immunotherapies such as immune checkpoint inhibitors. Further elucidation of the tumor mutational landscape has identified molecular targets for therapies, which have demonstrated success in other cancer types. In this review, we discuss both current and investigational immune and molecular targets of therapy for MCC. 相似文献
53.
54.
55.
56.
Drosophila suzukii is a significant pest of stone and small fruits. The genome of this species has been sequenced and manipulated by transposon‐mediated transformation and CRISPR/Cas9 gene editing. These technologies open a variety of possibilities for functional genomics and genetic modifications that might improve biologically based population control strategies. Both of these approaches, however, would benefit from genome targeting that would avoid position effects and insertional mutations associated with random transposon vector insertions, and the limited DNA fragment insertion size allowed by gene editing. Here, we describe an efficient recombinase‐mediated cassette exchange (RMCE) system for D. suzukii in which heterospecific lox recombination sites were integrated into the genome by transposon‐mediated transformation and subsequently targeted for double recombination by a donor vector in the presence of Cre recombinase. Three loxN/lox2272 landing site lines have previously been created in D. suzukii, and quantitative PCR determined that polyubiquitin‐regulated enhanced green fluorescent protein expression is least susceptible to position effect suppression in the 443_M26m1 line. We presume that RMCE target sites may also be inserted more specifically into the genome by homology‐directed repair gene editing, thereby avoiding position effects and mutations, while eliminating restrictions on the size of donor constructs for subsequent insertion. 相似文献
57.
58.
59.